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A new optimised methods to extract fish eDNA

Monitoring fish is necessary for understanding population dynamics, tracking distribution patterns and evaluating conservation efforts. Molecular techniques targeting environmental DNA (eDNA) are now considered effective methods for detecting specific species or characterising fish communities. The analysis of DNA from lake-surface sediments (sedDNA) can provide a time-integrated sampling approach which reduces the variability sometimes observed in water samples. However, studies of sedDNA have had varying success in detecting fish. The present study aimed to determine the most effective extraction method for recovering fish DNA from lake-surface sediments.

In the paper Optimised protocol for the extraction of fish DNA from freshwater sediments just published in Freshwater Biology members of the Lakes380 team, lead by PhD student Georgia Thomson-Laing, describe the development of a technique to maximise chances of extracting fish DNA from lake sediment.

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Sampling for fish eDNA in lakes

Check out these new eDNA posters! eDNA is an ama Check out these new eDNA posters! 

eDNA is an amazing new tool that can help us understand and protect the taiao (environment) - but it can't do everything. It needs to be used to complement other techniques and aligned with Indigenous and local knowledge. These new posters highlight these benefits and provide suggestions on some questions to ask before launching into an eDNA based project!

Email us for a high resolution PDF that you can print out. eDNA@cawthron.org.nz

When working with eDNA projects we like to co-design the project with our partners. We also love sharing our knowledge and helping train the next generation of scientists to become sentinels of the taiao.

#lake #eDNA #ecology #molecularecology #freshwater #lakes380 #cawthron #gnsscience #instaScience⁠ #NZscience
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About

  • The Project
  • The Science
    • Field Sampling
    • Laboratory Analysis
    • Student Projects
  • Science Aims
  • Rohe Studies
    • Rangitīkei Iwi Rohe Study
    • Wairarapa Moana iwi rohe study
  • Virtual Experience
  • Social Science
  • Iwi Relationships
  • The Team
    • Lakes380 Team
    • Advisory Groups
    • Lakes380 Co-lead Institutes​
    • National Collaborators
    • International Collaborators
  • Partners

Resources

  • Contact & Links
  • Blog, News & Publications
  • Video Gallery
  • Results
  • Glossary