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How long can sediment be stored for DNA analysis?

As part of Lakes380 and other large projects worldwide hundreds of sediment cores are being collected. Sometimes these are from places where it is not easy to store them chilled or because of the sheer numbers of cores collected they might get stored for weeks before sampling.

In this recently published study Katie Brasell and others assessed how long sediment could be stored in the fridge in the dark before the composition of microbes living in the sediment and DNA changed. The results showed that DNA yields and microbial community composition data shift significantly when sediments are stored chilled for more than 5 weeks. This highlights the need for rapid subsampling and appropriate storage of sediment core samples for DNA-based analysis.

You can read the full study here.

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Congratulations Jamie Howarth

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New manuscript on Rototoa (Auckland)

New manuscript on Lake Rototoa in Auckland has bee New manuscript on Lake Rototoa in Auckland has been published!!

A new manuscript titled - Resolving 500 years of anthropogenic impacts in a mesotrophic lake: Nutrients outweigh other drivers of lake change - led by Dr Rose Gregersen (Victoria University of Wellington) has been published in the prestigious journal Environmental Science & Technology.

The research explores the history of Rototoa, a lake in the Auckland region of Aotearoa-New Zealand. The researches combined sediment core analysis with historical data, and ecological experiments to determine what was causing the decline in lake health in recent decades. 

The researchers show that the health of the lake remained stable despite catchment deforestation and erosion, and the introduction of several non-native fish species. The most significant and sustained changes in lake health coincided with nitrogen and phosphorus fertilizers in the catchment.

Congratulations Rose on your excellent research!

Visit www.lakes380.com for a link to the full manuscript.

#Lakes380 #Cawthron #GNS #ScienceLife #NZscience #research #researchLife #VictoriaUniversityofWellington #Aucklandlakes #rototoa #paleolimnology #sedimentcores
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About

  • The Project
  • The Science
    • Field Sampling
    • Laboratory Analysis
    • Student Projects
  • Science Aims
  • Rohe Studies
    • Rangitīkei Iwi Rohe Study
    • Wairarapa Moana iwi rohe study
  • Virtual Experience
  • Social Science
  • Iwi Relationships
  • The Team
    • Advisory Groups
    • Lakes380 Co-lead Institutes​
    • National Collaborators
    • International Collaborators
  • Partners

Resources

  • Contact & Links
  • Blog, News & Publications
  • Video Gallery
  • Results
  • Glossary