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How long can sediment be stored for DNA analysis?

As part of Lakes380 and other large projects worldwide hundreds of sediment cores are being collected. Sometimes these are from places where it is not easy to store them chilled or because of the sheer numbers of cores collected they might get stored for weeks before sampling.

In this recently published study Katie Brasell and others assessed how long sediment could be stored in the fridge in the dark before the composition of microbes living in the sediment and DNA changed. The results showed that DNA yields and microbial community composition data shift significantly when sediments are stored chilled for more than 5 weeks. This highlights the need for rapid subsampling and appropriate storage of sediment core samples for DNA-based analysis.

You can read the full study here.

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Sampling for fish eDNA in lakes

Check out these new eDNA posters! eDNA is an ama Check out these new eDNA posters! 

eDNA is an amazing new tool that can help us understand and protect the taiao (environment) - but it can't do everything. It needs to be used to complement other techniques and aligned with Indigenous and local knowledge. These new posters highlight these benefits and provide suggestions on some questions to ask before launching into an eDNA based project!

Email us for a high resolution PDF that you can print out. eDNA@cawthron.org.nz

When working with eDNA projects we like to co-design the project with our partners. We also love sharing our knowledge and helping train the next generation of scientists to become sentinels of the taiao.

#lake #eDNA #ecology #molecularecology #freshwater #lakes380 #cawthron #gnsscience #instaScience⁠ #NZscience
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About

  • The Project
  • The Science
    • Field Sampling
    • Laboratory Analysis
    • Student Projects
  • Science Aims
  • Rohe Studies
    • Rangitīkei Iwi Rohe Study
    • Wairarapa Moana iwi rohe study
  • Virtual Experience
  • Social Science
  • Iwi Relationships
  • The Team
    • Lakes380 Team
    • Advisory Groups
    • Lakes380 Co-lead Institutes​
    • National Collaborators
    • International Collaborators
  • Partners

Resources

  • Contact & Links
  • Blog, News & Publications
  • Video Gallery
  • Results
  • Glossary